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For SDS-PAGE, choose from prestained and unstained, natural or recombinant protein ladders for broad, high, and low molecular weight ranges. Improved Dual Color Standards are brighter than the competition on both gels and blots. Bio-Rad protein ladders have been formulated and combined for use as molecular weight markers in a variety of applications including SDS-PAGE, IEF, 2-D PAGE, and western blotting. Increased overall brightness provides visual robustness throughout the entire protein electrophoresis and western blotting workflow. These standards offer absolute molecular weight accuracy confirmed by mass. Precision Plus Protein Unstained Standards contain the unique Strep -tag affinity peptide, which allows detection and molecular weight determination on western blots. 8 blue-stained bands and 2 pink reference bands (25 and 75 kD) These unstained protein standards include three high-intensity reference bands (25, 50, and 75 kD).Rad Laboratories, Clinical Division, 1000 Alfred Nobel Drive. Please use the filters below to select the specific protocol that best fits your needs. Here are a series of protocols, each explicit to a specific combination of reagents, detection modes, and laboratory equipment. Mixture of 10 recombinant proteins of precise molecular weights (10–250 kD) Precision Plus Protein Dual Color Standards from Bio-Rad. Western Blot ( WB ), and IFA, as well as for. Use Precision Plus Protein WesternC Standards as a combination standard for western blotting and fluorescence detection in SDS-PAGE. We know that western blotting protocols vary depending on your particular laboratory set-up and reagents of choice.Use improved Precision Plus Protein Dual Color Standards for molecular weight estimation on SDS-PAGE gels and western blots. Not intended for human, animal diagnostic or therapeutic uses.Place this handy selection guide magnet on your refrigerator or lab bench to eliminate the guesswork and make protein standard selection simple. The apparent molecular weight (kDa) of each protein has been determined by calibration against unstained protein standards supplemental data should be considered for more accurate adjustment in different electrophoresis conditions. Far western blotting (WB) was derived from the standard WB method to detect proteinprotein interactions in vitro. Additional data should be considered for a more accurate adjustment.Īpproximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate, pH 7.5 at 25☌), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol. Precision Plus Protein WesternC Standards are the recommended replacement products. Note: The molecular weight of each protein (kDa) was measured against an unstained protein ladder in every electrophoresis condition. Discontinued Product Biotinylated Natural SDS-PAGE Standards have been discontinued. Protein Ladders and Standards Precast Protein Gels Protein Electrophoresis and Blotting Products Supporting Documents and Resources. 2.5 μl per well for general Western transferring. Refer to the Blu13 (BLUelf) Prestained Protein Ladder patterns in various electrophoresis conditions:ģ μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. Guide for Molecular Weight Estimation (kDa) Use Precision Plus Protein WesternC Standards as a combination standard for western blotting and fluorescence detection in SDS-PAGE. The Blu13 Prestained Protein Ladder (BLUelf) keeps track of the size and separation of proteins during SDS-polyacrylamide gel electrophoresis, approximating protein size and validating Western transfer efficiency on PVDF, nylon, or nitrocellulose membranes. Unstained Natural Protein Standards for SDS-PAGE are blended to give uniform band intensities when stained with Coomassie Blue R-250 or zinc stains.
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The 13 recombinant proteins are covalently coupled with blue chromophore, while 1 green band at 25 kDa and a red band at 75 kDa serve as reference bands. is licensed by Institut für Bioanalytik GmbH to sell these products for research use only. StrepTactin is covered by German patent application P 19641876.3. The Blu13 Prestained Protein Ladder (BLUelf) is a combination of 13 pre-stained proteins with molecular weights from 5 to 245 kDa. Strep-tag technology for western blot detection is covered by US patent 5,506,121 and by UK patent 2,272,698. Following SDS-PAGE, transfer proteins onto blotting membrane according to the manufacturer’s instructions.